Aim
To
know the volume lung of insects on the rate of respiration.
Preliminary
Insects
Air enters the respiratory
systems of most insects through a series of external openings called spiracles.
These external openings, which act as muscular valves in some insects, lead to
the internal respiratory system, a densely networked array of tubes called
tracheae. The scientific tracheal system within an individual is composed of
interconnecting transverse and longitudinal tracheae which maintain equivalent
pressure throughout the system. These tracheae branch repeatedly, eventually
forming tracheoles, which are blind-ended, water-filled compartments only one
micrometer in diameter. It is at this level of the tracheoles that oxygen is
delivered to the cells for respiration. The trachea are water-filled due to the
permeable membrane of the surrounding tissues. During exercise, the water level
retracts due to the increase in concentration of lactic acid in the muscle
cells. This lowers the water potential and the water is drawn back into the
cells via osmosis and air is brought closer to the muscle cells. The diffusion
pathway is then reduced and gases can be transferred more easily.
Insects were once believed to
exchange gases with the environment continuously by the simple diffusion of
gases into the tracheal system. More recently, however, large variation in
insect ventilatory patterns have been documented and insect respiration appears
to be highly variable. Some small insects do demonstrate continuous respiration
and may lack muscular control of the spiracles. Others, however, utilize
muscular contraction of the abdomen along with coordinated spiracle contraction
and relaxation to generate cyclical gas exchange patterns and to reduce water loss
into the atmosphere. The most extreme form of these patterns is termed
discontinuous gas exchange cycles (DGC).
·
Respirometre
·
Balance
·
Pipette
·
Vaseline
·
NaOH crystal
·
Eosine solution
·
Tissue
·
4 Crickets
·
Spatula
Step
Ø Wrap
the NaOH crystal with cotton and insert into the respirometre tube,
Ø Weight
and measure the cricket,
Ø Insert
the cricket into the respirometre tube and close with a pipe that has been
covered with vaseline on the surface,
Ø Cover
the end of the pipe with your finger for one minute then release your finger
and insert a drop of eosin using a pipette,
Ø Look
and write on note the changes occuring on the position of the eosin drop in the
pipe every 1 minutes for 4 minutes,
Ø Repeat
the test using a different cricket until 4 crickets.
Observation Data
|
Number
|
Weight of cricket (gr)
|
Volume descrese (ml) of 2 minute period.
.
|
Total of volume
|
|
1
|
2
|
3
|
4
|
|
1
|
0.45
|
0.07
|
0.18
|
0.18
|
0.13
|
0.56
|
|
2
|
0.56
|
0.08
|
0.11
|
0.12
|
0.12
|
0.43
|
|
3
|
0.77
|
0.11
|
0.09
|
0.1
|
0.07
|
0.37
|
|
4
|
0.55
|
0.18
|
0.19
|
0.12
|
0.07
|
0.56
|
gram
1
0,8
0,4
0,2
0
0,37 0,43
0,56
Data Analysis
1. Explain
why during the expiration sampling the air needs to be released 100 ml ?
To
lose the atmosphere air pressure
2. Based
on your measurements, explain the content of oxygen in the atmosphere and after
respiration ? O2 being to HgO2 and will be changed to CO2
in exhale.
3. What
factor causes the eosin drop to change position ?
Because
there is a cricket in the respirometre tube that it inhale 02 from
respiro, but the eosin it closed it and eosin is the measurement.
Factor
: the weight of body, temperature, and types of insects
4. Make
a conclusion based on the graphic you had done ?
From
my data, the heavier the cricket,
the less need of oxygen.
My
friends said “The more cricket body weight, the more requires oxygen. While the lighter weight of a
cricket body needs less oxygen. Like humans, when human more fat, they require more oxygen.”
But I not agree because more weight the body of the living thing it’s maked the
lung is bigger, and the inhale is very much at first. But the save air in lung
maked the living thing less inhale. May
be at the time of trial, we made a mistake or do not accurately
measure the weight of the cricket sand see the changes in the position of eosin.
Because the respirometre is lay not stand up.
Conclusion
From
my data, the heavier the cricket,
the less need of oxygen. more weight the
body of the living thing it’s maked the lung is bigger, and the inhale is very
much at first. But the save air in lung maked the living thing less inhale May
be at the time of trial, we made a
mistake or do not accurately measure the weight of the cricket sand see the changes
in the position of eosin. Because the respirometre is lay not stand up.
Bibliography
·
http://rifziest.blogspot.com/2009/08/laporan-percobaan-respirasi-menggunaka.html
·
http://secondscienceedu.wordpress.com/ceedu-junior-high/laporan-praktikum-biologi-%E2%80%9Crespirasi-serangga%E2%80%9D/
